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MeV Nucleoprotein Recombinant Protein (N-His) (VK397012)

MeV Nucleoprotein Recombinant Protein (N-His)
MeV Nucleoprotein Recombinant Protein (N-His)
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Overview
Catalog No.VK397012
Description
Recombinant MeV Nucleoprotein, N-His (VK397012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionQ77M43
Protein lengthThr21-Arg408
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesMeasles virus (strain Edmonston-Moraten vaccine) (MeV)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesN, NP, Nucleocapsid protein, Nucleoprotein, Protein N
Background

Nucleoprotein is a ~58 kDa protein. Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter. The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P.

1. Desfosses, A. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 4256-4264. PMID: 30787192
2. Zhou, Y. et al. (2019) Journal of virology 93. PMID: 31375591
Note For research use only
Images
  • MeV Nucleoprotein Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant MeV N/Nucleoprotein Protein, N-His

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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