Human PPP6C Recombinant Protein (N-His) (HT366012)

Name: Human PPP6C Recombinant Protein (N-His)
Brand: abinScience
SKU: HT366012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HT366012
Description	Recombinant Human PPP6C Protein, N-His (HT366012) expressed in E. coli, spanning Met1-Leu305. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	O00743
Protein length	Met1-Leu305
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.31 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Serine/threonine-protein phosphatase 6 catalytic subunit, PP6C, 3.1.3.16, Serine/threonine-protein phosphatase 6 catalytic subunit, N-terminally processed, PPP6C, PPP6
Background	Serine/threonine-protein phosphatase 6 catalytic subunit (PPP6C) is a ~35 kDa protein. Catalytic subunit of protein phosphatase 6 (PP6). PP6 is a component of a signaling pathway regulating cell cycle progression in response to IL2 receptor stimulation. N-terminal domain restricts G1 to S phase progression in cancer cells, in part through control of cyclin D1. During mitosis, regulates spindle positioning. Down-regulates MAP3K7 kinase activation of the IL1 signaling pathway by dephosphorylation of MAP3K7. 1. Kajino, T. et al. (2006) The Journal of biological chemistry 281, 39891-6. PMID: 17079228 2. Tan, P. et al. (2017) Molecular cell 68, 293-307.e5. PMID: 29053956 3. Li, M. et al. (2020) Protein & cell 11, 584-599. PMID: 32474700 4. Filali, M. et al. (1999) Journal of cellular biochemistry 73, 153-63. PMID: 10227379 5. Stefansson, B. et al. (2007) Cell cycle (Georgetown, Tex.) 6, 1386-92. PMID: 17568194 6. Kotak, S. et al. (2016) Journal of cell science 129, 3015-25. PMID: 27335426 7. Ni, G. et al. (2020) mBio 11. PMID: 32753499
Note	For research use only.

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SDS-PAGE for Recombinant Human PPP6C Protein, N-His

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Datasheet

Human PPP6C Recombinant Protein (N-His).pdf

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Human PPP6C Recombinant Protein (N-His) (HT366012)

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