ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
25.38 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Leukotriene A-4 hydrolase (LTA4H) is a ~69 kDa protein. Bifunctional zinc metalloenzyme that comprises both epoxide hydrolase (EH) and aminopeptidase activities. Acts as an epoxide hydrolase to catalyze the conversion of LTA4 to the pro-inflammatory mediator leukotriene B4 (LTB4). Can utilize LTA5 less effectively as a substrate than LTA4, and produce LTB5. Also has aminopeptidase activity, with high affinity for N-terminal arginines of various synthetic tripeptides. In addition to its pro-inflammatory EH activity, may also counteract inflammation by its aminopeptidase activity, which inactivates by cleavage another neutrophil attractant, the tripeptide Pro-Gly-Pro (PGP), a bioactive fragment of collagen generated by the action of matrix metalloproteinase-9 (MMP9) and prolylendopeptidase (PREPL).
1. Rudberg, PC. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 4215-20. PMID: 11917124
2. Thunnissen, MM. et al. (2002) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 16, 1648-50. PMID: 12207002
3. Rudberg, PC. et al. (2004) The Journal of biological chemistry 279, 27376-82. PMID: 15078870
4. Tholander, F. et al. (2008) Chemistry & biology 15, 920-9. PMID: 18804029
5. Odlander, B. et al. (1991) Archives of biochemistry and biophysics 287, 167-74. PMID: 1897988
6. Toh, H. et al. (1990) Biochemical and biophysical research communications 171, 216-21. PMID: 1975494
7. Haeggström, JZ. et al. (1990) Biochemical and biophysical research communications 172, 965-70. PMID: 2244921
8. Nathaniel, DJ. et al. (1985) Biochemical and biophysical research communications 131, 827-35. PMID: 2996528
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human LTA4H Protein, N-His
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