Human KANK2 Recombinant Protein (N-His) (HA852012)

Name: Human KANK2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA852012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HA852012
Description	Recombinant Human KANK2 Protein, N-His (HA852012) expressed in E. coli, spanning Met1-Asn161. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q63ZY3
Protein length	Met1-Asn161
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	21.00 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Ankyrin repeat domain-containing protein 25, SRC-interacting protein, SRC-1-interacting protein, SRC1-interacting protein, ANKRD25, KIAA1518, MXRA3, KN motif and ankyrin repeat domain-containing protein 2, Matrix-remodeling-associated protein 3, KANK2, SIP
Background	KN motif and ankyrin repeat domain-containing protein 2 (KANK2) is a ~91 kDa protein. Involved in transcription regulation by sequestering in the cytoplasm nuclear receptor coactivators such as NCOA1, NCOA2 and NCOA3. Involved in regulation of caspase-independent apoptosis by sequestering the proapoptotic factor AIFM1 in mitochondria. Pro-apoptotic stimuli can induce its proteasomal degradation allowing the translocation of AIFM1 to the nucleus to induce apoptosis. Involved in the negative control of vitamin D receptor signaling pathway. Involved in actin stress fibers formation through its interaction with ARHGDIA and the regulation of the Rho signaling pathway. 1. Zhang, Y. et al. (2007) The EMBO journal 26, 2645-57. PMID: 17476305 2. Wang, D. et al. (2012) The Journal of biological chemistry 287, 12612-21. PMID: 22371500 3. Ramot, Y. et al. (2014) Journal of medical genetics 51, 388-94. PMID: 24671081 4. Zhu, Y. et al. (2008) Biochimica et biophysica acta 1780, 128-33. PMID: 17996375 5. Gee, HY. et al. (2015) The Journal of clinical investigation 125, 2375-84. PMID: 25961457
Note	For research use only.

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SDS-PAGE for Recombinant Human KANK2 Protein, N-His

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Datasheet

Human KANK2 Recombinant Protein (N-His).pdf

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Human KANK2 Recombinant Protein (N-His) (HA852012)

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