SARS-CoV-2 ORF8/ns8 Recombinant Protein (N-His-SUMO) (VK460022)

Name: SARS-CoV-2 ORF8/ns8 Recombinant Protein (N-His-SUMO)
Brand: abinScience
SKU: VK460022
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	VK460022
Description	Recombinant SARS-CoV-2 ORF8/ns8 Protein, N-His-SUMO (VK460022) expressed in E. coli, spanning Phe16-Ile121. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P0DTC8
Protein length	Phe16-Ile121
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	24.75 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ORF8 protein, ORF8, Non-structural protein 8, ns8
Background	ORF8 protein (ns8) is a ~13 kDa protein. Plays a role in modulating the host immune response. May act as a secreted virokine by mimicking interleukin-17A (IL17A), and thereby binding to the IL17RA receptor, leading to activation of the IL17 pathway and increased secretion of pro-inflammatory factors. Contributes to the cytokine storm during SARS-CoV-2 infection when secreted by unconventional pathway. May act by down-regulating major histocompability complex class I (MHC-I) at cell surface. May inhibit expression of some members of the IFN-stimulated gene (ISG) family including hosts IGF2BP1/ZBP1, MX1 and MX2, and DHX58. 1. Chan, JF. et al. (2020) Lancet (London, England) 395, 514-523. PMID: 31986261 2. Wu, X. et al. (2022) mBio 13, e0040222. PMID: 35343786 3. Lin, X. et al. (2023) PLoS pathogens 19, e1011128. PMID: 36689483 4. Lin, X. et al. (2021) iScience 24, 102293. PMID: 33723527 5. Zhang, Y. et al. (2021) Proceedings of the National Academy of Sciences of the United States of America 118. PMID: 34021074 6. Matsuoka, K. et al. (2022) The Journal of biological chemistry 298, 101724. PMID: 35157849 7. Geng, H. et al. (2021) Frontiers in immunology 12, 679482. PMID: 34177923
Note	For research use only.