ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
25.69 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Prelamin-A/C is a ~74 kDa protein. Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. Lamin A and C are present in equal amounts in the lamina of mammals.
1. Bonne, G. et al. (1999) Nature genetics 21, 285-8. PMID: 10080180
2. Fatkin, D. et al. (1999) The New England journal of medicine 341, 1715-24. PMID: 10580070
3. Cao, H. et al. (2000) Human molecular genetics 9, 109-12. PMID: 10587585
4. Muchir, A. et al. (2000) Human molecular genetics 9, 1453-9. PMID: 10814726
5. De Sandre-Giovannoli, A. et al. (2002) American journal of human genetics 70, 726-36. PMID: 11799477
6. Novelli, G. et al. (2002) American journal of human genetics 71, 426-31. PMID: 12075506
7. Chen, L. et al. (2003) Lancet (London, England) 362, 440-5. PMID: 12927431
8. Navarro, CL. et al. (2004) Human molecular genetics 13, 2493-503. PMID: 15317753
9. Quijano-Roy, S. et al. (2008) Annals of neurology 64, 177-86. PMID: 18551513
10. Renou, L. et al. (2008) Journal of medical genetics 45, 666-71. PMID: 18611980
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human LMNA Protein, N-His
中文
English
한국어
日本語
Español
Français
Русский
