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Recombinant Human IL11 Protein, N-GST & C-His (HB978032)

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Recombinant Human IL11 Protein, N-GST & C-His

Recombinant Human IL11 Protein, N-GST & C-His

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Datasheet

Overview

Catalog No.	HB978032
Description	Recombinant Human IL11 Protein, N-GST & C-His (HB978032) expressed in E. coli, spanning Pro22-Leu199. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P20809
Protein length	Pro22-Leu199
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	47.33 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Interleukin-11, IL-11, Adipogenesis inhibitory factor, AGIF, Oprelvekin, IL11
Background	Interleukin-11 is a ~21 kDa protein. Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. Also promotes the proliferation of hepatocytes in response to liver damage. Binding to its receptor formed by IL6ST and IL11RA activates a signaling cascade that promotes cell proliferation. Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3. The interaction with the membrane-bound IL11RA and IL6ST stimulates 'classic signaling', whereas the binding of IL11 and soluble IL11RA to IL6ST stimulates 'trans-signaling'. 1. Paul, SR. et al. (1990) Proceedings of the National Academy of Sciences of the United States of America 87, 7512-6. PMID: 2145578 2. Harmegnies, D. et al. (2003) The Biochemical journal 375, 23-32. PMID: 12919066 3. Lamertz, L. et al. (2018) Science signaling 11. PMID: 30279168
Note	For research use only

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Bioactivity

Detected by Research Grade Anti-Human IL11 Antibody (X203) (Catalog No: HB978016) in indirect ELISAs.

References

Datasheet

Recombinant Human IL11 Protein, N-GST & C-His.pdf

Formula

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Recombinant Human IL11 Protein, N-GST & C-His