ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) (MERS-CoV)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
39.16 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
Replicase polyprotein 1a, pp1a, ORF1a polyprotein, Host translation inhibitor nsp1, nsp1, Leader protein, Non-structural protein 2, nsp2, p65 homolog, Papain-like protease nsp3, PL-PRO, EC:3.4.19.12, EC:3.4.22.-, Non-structural protein 3, nsp3, PL2-PRO, Non-structural protein 4, nsp4, 3C-like proteinase nsp5, 3CL-PRO, 3CLp, EC:3.4.22.69, nsp5, Non-structural protein 6, nsp6, Non-structural protein 7, nsp7, Non-structural protein 8, nsp8, RNA-capping enzyme subunit nsp9, Non-structural protein 9, nsp9, EC:2.7.7.50, Non-structural protein 10, nsp10, Growth factor-like peptide, GFL, Non-structural protein 11, nsp11
Background
Replicase polyprotein 1a is a ~486 kDa protein. The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.
Note
For research use only
Images
SDS-PAGE
SDS-PAGE for Recombinant MERS-CoV nsp3 Protein, N-His
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