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Arabidopsis thaliana CARK1 Recombinant Protein (N-His) (PR011012)

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Overview
Catalog No.PR011012
Description
Recombinant Arabidopsis thaliana CARK1 Protein, N-His (PR011012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionQ9LUT0
Protein lengthGln50-Asn350
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesArabidopsis thaliana (Mouse-ear cress)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesCARK1, EC:2.7.11.1, Protein CYTOSOLIC ABA RECEPTOR KINASE 1, Receptor-like cytoplasmic kinase 1
Background

Protein CYTOSOLIC ABA RECEPTOR KINASE 1 is a ~39 kDa protein. Receptor-like cytoplasmic kinase (RLCK) that triggers abscisic acid (ABA) signaling in response to dehydration by phosphorylating and activating ABA receptors (e.g. PYL8/RCAR3 and PYR1/RCAR11) to support their monomerization and enhance their ABA binding affinity; they in turn repress PP2C protein phosphatases (e.g. ABI1) acting as negative regulators of ABA responses. Promotes drought tolerance.

1. Zhang, L. et al. (2018) Cell discovery 4, 30. PMID: 29928509
2. Li, X. et al. (2022) The New phytologist 235, 533-549. PMID: 35388459
3. Ge, H. et al. (2018) International journal of molecular sciences 19. PMID: 29970817
Note For research use only
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References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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