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Human CEP250 Recombinant Protein (N-His) (HD660012)

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Overview
Catalog No.HD660012
Description
Recombinant Human CEP250 Protein, N-His (HD660012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionQ9BV73
Protein lengthArg40-Thr293
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesHomo sapiens (Human)
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names250 kDa centrosomal protein, C-Nap1, CEP2, CEP250, CNAP1, Centrosomal Nek2-associated protein 1, Centrosomal protein 2, Centrosome-associated protein CEP250, Cep250
Background

Centrosome-associated protein CEP250 is a ~281 kDa protein. Plays an important role in centrosome cohesion during interphase. Recruits CCDC102B to the proximal ends of centrioles. Maintains centrosome cohesion by forming intercentriolar linkages. Accumulates at the proximal end of each centriole, forming supramolecular assemblies with viscous material properties that promote organelle cohesion. May be involved in ciliogenesis.

1. Xia, Y. et al. (2018) Journal of cell science 131. PMID: 30404835
2. Mahen, R. (2022) PLoS biology 20, e3001854. PMID: 36282799
3. de Castro-Miró, M. et al. (2016) PloS one 11, e0168966. PMID: 28005958
Note For research use only
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References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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