| Catalog No. | HA183012 |
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| Expression system | E. coli |
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| Accession | Q9BY67 |
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| Protein length | Asn46-Tyr331 |
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| Applications | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
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| Species | Homo sapiens (Human) |
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| Nature |
Recombinant |
| Endotoxin level |
Please contact with the lab for this information. |
| Purity |
>90% as determined by SDS-PAGE. |
| Predicted molecular weight |
34.80 kDa |
| Form |
Lyophilized |
| Storage buffer |
Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
|
| Reconstitution |
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Shipping |
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Stability and Storage |
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Alternate Names | CADM1, Cell adhesion molecule 1, IGSF4, IGSF4A, IgSF4, Immunoglobulin superfamily member 4, NECL-2, NECL2, Nectin-like protein 2, SYNCAM, SgIgSF, Spermatogenic immunoglobulin superfamily, SynCAM, Synaptic cell adhesion molecule, TSLC-1, TSLC1, Tumor suppressor in lung cancer 1 |
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| Background | Cell adhesion molecule 1 is a ~48 kDa protein. Mediates homophilic cell-cell adhesion in a Ca(2+)-independent manner. Also mediates heterophilic cell-cell adhesion with CADM3 and NECTIN3 in a Ca(2+)-independent manner. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM1 in vivo. In mast cells, may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. 1. Masuda, M. et al. (2002) The Journal of biological chemistry 277, 31014-9. PMID: 120501602. Moiseeva, EP. et al. (2012) Cellular and molecular life sciences : CMLS 69, 2751-64. PMID: 224380594. Furuno, T. et al. (2005) Journal of immunology (Baltimore, Md. : 1950) 174, 6934-42. PMID: 159055365. Kuramochi, M. et al. (2001) Nature genetics 27, 427-30. PMID: 112795266. Yageta, M. et al. (2002) Cancer research 62, 5129-33. PMID: 122349737. Ito, A. et al. (2003) Laboratory investigation; a journal of technical methods and pathology 83, 1175-83. PMID: 12920246 |
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| Note |
For research use only |
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