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Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056) (YP205015)

Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056)
Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056)
Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056)
Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056)
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Overview
Catalog No.YP205015
Description
Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056) [1A056] (YP205015) is a mouse monoclonal antibody detecting 6*His Tag in ELISA, IP, WB. Suitable for General.
Highlights
  • Multi-Application — Validated across multiple applications.
Species reactivityGeneral
ApplicationsELISA, IP, WB
Host speciesMouse
IsotypeIgG1
Clone ID1A056
Clonality Monoclonal
Immunogen Synthetic peptide HHHHHH-conjugated to KLH.
Target 6*His Tag, His Tag, His-Tag, 6*His Tag
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056)

    SDS-PAGE

    SDS-PAGE for Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056)

  • Anti-6*His Tag (HHHHHH) Monoclonal Antibody (1A056)

    Western blot

    Western blot analysis was performed using anti-6*His tag monoclonal antibody at 1ug/mL on various samples.
    Lane 1: Recombinant Human APMAP Protein, N-His (Catalog No. HV613012), 100 ng
    Lane 2: Recombinant Human DDX20 Protein, N-His (Catalog No. HP319012), 100 ng

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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