Anti-ABCA1 Polyclonal Antibody (HF602014)

Name: Anti-ABCA1 Polyclonal Antibody
Brand: abinScience
SKU: HF602014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF602014

Description

Anti-ABCA1 Polyclonal Antibody (HF602014) is a rabbit polyclonal antibody detecting ABCA1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ABCA1 (Ser45-Glu271).

Target

ATP-binding cassette transporter 1, ATP-binding cassette sub-family A member 1, Phospholipid-transporting ATPase ABCA1, ABC-1, ABCA1, ATP-binding cassette 1, Cholesterol efflux regulatory protein, ABC1, CERP

Purification

Purified by antigen affinity column.

Accession

O95477

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Phospholipid-transporting ATPase ABCA1 is a ~254 kDa protein. Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Thereby, participates in phospholipid transfer to apolipoproteins to form nascent high density lipoproteins/HDLs. Transports preferentially phosphatidylcholine over phosphatidylserine. May play a similar role in the efflux of intracellular cholesterol to apolipoproteins and the formation of nascent high density lipoproteins/HDLs. Translocates phospholipids from the outer face of the plasma membrane and forces it through its gateway and annulus into an elongated hydrophobic tunnel in its extracellular domain.

1. Quazi, F. et al. (2013) The Journal of biological chemistry 288, 34414-26. PMID: 24097981

2. Segrest, JP. et al. (2022) Nature communications 13, 4812. PMID: 35974019

3. Krimbou, L. et al. (2004) Journal of lipid research 45, 839-48. PMID: 14754908

4. Marcil, M. et al. (1999) Lancet (London, England) 354, 1341-6. PMID: 10533863

Note

For research use only.

Images

References

Datasheet

Anti-ABCA1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ABCA1 Polyclonal Antibody (HF602014)

Overview

Images

References

Datasheet

SDS

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