Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339) (HX034013)

Name: Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)
Brand: abinScience
SKU: HX034013
Price: 76 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HX034013

Description

Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339) (HX034013) is a mouse monoclonal antibody detecting ACTB/β-actin in ELISA, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

SAA0339

Target

Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB

Endotoxin level

< 10 EU/mg

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Actin, cytoplasmic 1 (ACTB/β-actin) is a ~41 kDa protein. Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA. Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments. Part of the ACTR1A/ACTB filament around which the dynactin complex is built.

1. Hundt, N. et al. (2014) The FEBS journal 281, 5279-91. PMID: 25255767

2. Drazic, A. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 4399-4404. PMID: 29581253

3. Schrank, BR. et al. (2018) Nature 559, 61-66. PMID: 29925947

4. Serna, M. et al. (2024) Developmental cell 59, 3175-3188.e8. PMID: 39321809

5. Aher, A. et al. (2024) Nature structural & molecular biology 31, 1124-1133. PMID: 38609661

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-ACTB/β-actin/Beta Actin Antibody (SAA0339)
Western blot

Western blot analysis was performed using anti-beta Actin monoclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: NIH3T3 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: Mouse brain lysate
Lane 5: Rat brain lysate
Lane 6: Mouse spleen lysate
Lane 7: Rat spleen lysate
Western blot

Western blot analysis was performed using anti-beta actin monoclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: 293T cell lysate
Lane 3: K562 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Mouse brain lysate
Lane 6: Rat brain lysate

References

Datasheet

Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339) (HX034013)

Overview

Images

References

Datasheet

SDS