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Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339) (HX034013)

Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)
Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)
Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)
Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)
Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)
Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)
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  • Overview

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Overview
Catalog No.HX034013
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, WB
Host speciesMouse
IsotypeIgG2a, kappa
Clone IDSAA0339
Clonality Monoclonal
Target Actin, cytoplasmic 1, Beta-actin, Actin, cytoplasmic 1, N-terminally processed, ACTB
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Images
  • Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)

    SDS-PAGE

    SDS-PAGE for Anti-ACTB/β-actin/Beta Actin Antibody (SAA0339)

  • Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)

    Western blot

    Western blot analysis was performed using anti-beta Actin monoclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: NIH3T3 cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: Mouse brain lysate
    Lane 5: Rat brain lysate
    Lane 6: Mouse spleen lysate
    Lane 7: Rat spleen lysate

  • Anti-ACTB/β-actin/Beta Actin Recombinant Antibody (SAA0339)

    Western blot

    Western blot analysis was performed using anti-beta actin monoclonal antibody at 1ug/mL on various samples.
    Lane 1: Hela cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate
    Lane 4: PC-12 cell lysate
    Lane 5: Mouse brain lysate
    Lane 6: Rat brain lysate

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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