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Anti-AFM Polyclonal Antibody (HW796014)

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Overview
Catalog No.HW796014
Description
Anti-AFM Polyclonal Antibody (HW796014) is a rabbit polyclonal antibody detecting AFM in ELISA, IHC, WB. Suitable for Human.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human AFM (Leu22-Thr210).
Target ALB2, Alpha-Alb, AFM, Alpha-albumin, ALBA, Afamin
Purification Purified by antigen affinity column.
Accession P43652
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Afamin (AFM) is a ~69 kDa protein. Functions as a carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.

1. Mihara, E. et al. (2016) eLife 5. PMID: 26902720
2. Voegele, AF. et al. (2002) Biochemistry 41, 14532-8. PMID: 12463752
3. Jerkovic, L. et al. (2005) Journal of proteome research 4, 889-99. PMID: 15952736
4. Kratzer, I. et al. (2009) Journal of neurochemistry 108, 707-18. PMID: 19046407
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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