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Anti-Human AFM/Afamin Recombinant Antibody (N14) (HW796013)

Anti-Human AFM/Afamin Recombinant Antibody (N14)
Anti-Human AFM/Afamin Recombinant Antibody (N14)
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Overview
Catalog No.HW796013
Species reactivityHuman
ApplicationsELISA
Host speciesMouse
IsotypeIgG1
Clone IDN14
Clonality Monoclonal
Target ALB2, Alpha-Alb, AFM, Alpha-albumin, ALBA, Afamin
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P43652
RRID Anti-Human AFM/Afamin Antibody (N14) (abinScience Cat# HW796013, RRID:AB_3726960)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Afamin (AFM) is a ~69 kDa protein. Functions as a carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.

1. Mihara, E. et al. (2016) eLife 5. PMID: 26902720
2. Voegele, AF. et al. (2002) Biochemistry 41, 14532-8. PMID: 12463752
3. Jerkovic, L. et al. (2005) Journal of proteome research 4, 889-99. PMID: 15952736
4. Kratzer, I. et al. (2009) Journal of neurochemistry 108, 707-18. PMID: 19046407
Note For research use only.
Images
  • Anti-Human AFM/Afamin Recombinant Antibody (N14)

    SDS-PAGE

    SDS-PAGE for Anti-Human AFM/Afamin Antibody (N14).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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