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Anti-Human AFM/Afamin Recombinant Antibody (N14) (HW796013)

Anti-Human AFM/Afamin Recombinant Antibody (N14)
Anti-Human AFM/Afamin Recombinant Antibody (N14)
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Overview
Catalog No.HW796013
Description
Anti-Human AFM/Afamin Recombinant Antibody (N14) (HW796013) is a mouse monoclonal antibody detecting AFM/Afamin in ELISA. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
Species reactivityHuman
ApplicationsELISA
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1, kappa
Clone IDN14
Target ALB2, Alpha-Alb, AFM, Alpha-albumin, ALBA, Afamin
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P43652
RRID Anti-Human AFM/Afamin Antibody (N14) (abinScience Cat# HW796013, RRID:AB_3726960)
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Afamin (AFM) is a ~69 kDa protein. Functions as a carrier for hydrophobic molecules in body fluids (Probable). Essential for the solubility and activity of lipidated Wnt family members, including WNT1, WNT2B, WNT3, WNT3A, WNT5A, WNT7A, WNT7B, WNT8, WNT9A, WNT9B, WNT10A and WNT10B. Binds vitamin E. May transport vitamin E in body fluids under conditions where the lipoprotein system is not sufficient. May be involved in the transport of vitamin E across the blood-brain barrier.

1. Mihara, E. et al. (2016) eLife 5. PMID: 26902720
2. Voegele, AF. et al. (2002) Biochemistry 41, 14532-8. PMID: 12463752
3. Jerkovic, L. et al. (2005) Journal of proteome research 4, 889-99. PMID: 15952736
4. Kratzer, I. et al. (2009) Journal of neurochemistry 108, 707-18. PMID: 19046407
Note For research use only.
Isotype Control Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21) (MV080207)
Images
  • Anti-Human AFM/Afamin Recombinant Antibody (N14)

    SDS-PAGE

    SDS-PAGE for Anti-Human AFM/Afamin Antibody (N14).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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