Anti-ALOX12B Polyclonal Antibody (HF742014)

Name: Anti-ALOX12B Polyclonal Antibody
Brand: abinScience
SKU: HF742014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HF742014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ALOX12B (Leu479-Ile701).

Target

ALOX12B, Epidermis-type lipoxygenase 12, Arachidonate 12-lipoxygenase, 12R-type, 12R-LOX, 12R-lipoxygenase

Purification

Purified by antigen affinity column.

Accession

O75342

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Arachidonate 12-lipoxygenase, 12R-type (ALOX12B) is a ~80 kDa protein. Catalyzes the regio and stereo-specific incorporation of a single molecule of dioxygen into free and esterified polyunsaturated fatty acids generating lipid hydroperoxides that can be further reduced to the corresponding hydroxy species. In the skin, acts upstream of ALOXE3 on the lineolate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins. Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss. May also play a role in the regulation of the expression of airway mucins.

1. Zheng, Y. et al. (2011) The Journal of biological chemistry 286, 24046-56. PMID: 21558561

2. Boeglin, WE. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 6744-9. PMID: 9618483

3. Sun, D. et al. (1998) The Journal of biological chemistry 273, 33540-7. PMID: 9837935

4. Garcia-Verdugo, I. et al. (2012) The European respiratory journal 40, 714-23. PMID: 22441738

Note

For research use only.

Images

Immunofluorescence

A549 cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting ALOX12B (HF742014) at a concentration of 28 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of ALOX12B was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet

Anti-ALOX12B Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ALOX12B Polyclonal Antibody (HF742014)

Overview

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References

Datasheet

SDS

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