Anti-ANO6 Polyclonal Antibody (HS838014)

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100ug

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Overview

Catalog No.

HS838014

Description

Anti-ANO6 Polyclonal Antibody (HS838014) is a rabbit polyclonal antibody detecting ANO6 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ANO6 (Ala744-Asn824).

Target

SCAN channel, ANO6, TMEM16F, Small-conductance calcium-activated nonselective cation channel, Transmembrane protein 16F, Anoctamin-6

Purification

Purified by antigen affinity column.

Accession

Q4KMQ2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Anoctamin-6 (ANO6/TMEM16F) is a ~106 kDa protein. Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes.

1. Schreiber, R. et al. (2010) The Journal of biological chemistry 285, 7838-45. PMID: 20056604

2. Suzuki, J. et al. (2010) Nature 468, 834-8. PMID: 21107324

3. Kunzelmann, K. et al. (2012) Experimental physiology 97, 184-92. PMID: 21908539

4. Martins, JR. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 18168-72. PMID: 22006324

5. Tian, Y. et al. (2012) Journal of cell science 125, 4991-8. PMID: 22946059

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-ANO6 polyclonal antibody at 1ug/mL on recombinant ANO6 (Catalog No. HS838012).

References

Datasheet

Anti-ANO6 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ANO6 Polyclonal Antibody (HS838014)

Overview

Images

References

Datasheet

SDS

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