Human ANO6 Recombinant Protein (N-His) (HS838012)

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Overview

Catalog No.	HS838012
Description	Recombinant Human ANO6 Protein, N-His (HS838012) expressed in E. coli, spanning Ala744-Asn824. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q4KMQ2
Protein length	Ala744-Asn824
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	SCAN channel, ANO6, TMEM16F, Small-conductance calcium-activated nonselective cation channel, Transmembrane protein 16F, Anoctamin-6
Background	Anoctamin-6 (ANO6/TMEM16F) is a ~106 kDa protein. Small-conductance calcium-activated nonselective cation (SCAN) channel which acts as a regulator of phospholipid scrambling in platelets and osteoblasts. Phospholipid scrambling results in surface exposure of phosphatidylserine which in platelets is essential to trigger the clotting system whereas in osteoblasts is essential for the deposition of hydroxyapatite during bone mineralization. Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide. Can generate outwardly rectifying chloride channel currents in airway epithelial cells and Jurkat T lymphocytes. 1. Schreiber, R. et al. (2010) The Journal of biological chemistry 285, 7838-45. PMID: 20056604 2. Suzuki, J. et al. (2010) Nature 468, 834-8. PMID: 21107324 3. Kunzelmann, K. et al. (2012) Experimental physiology 97, 184-92. PMID: 21908539 4. Martins, JR. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 18168-72. PMID: 22006324 5. Tian, Y. et al. (2012) Journal of cell science 125, 4991-8. PMID: 22946059
Note	For research use only.

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SDS-PAGE for Recombinant Human ANO6 Protein, N-His

References

HS838012-SDS-PAGE-1.jpg

Datasheet

Human ANO6 Recombinant Protein (N-His).pdf

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Human ANO6 Recombinant Protein (N-His) (HS838012)

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