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Anti-ASFV B438L/p49 Polyclonal Antibody (VK754014)

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Overview
Catalog No.VK754014
Description
Anti-ASFV B438L/p49 Polyclonal Antibody (VK754014) is a rabbit polyclonal antibody detecting B438L in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityAfrican swine fever virus (ASFV)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant ASFV B438L/p49 (Ser257-Ser415).
Target Minor capsid protein p49, p49, Ba71V-075, B438L
Purification Purified by antigen affinity column.
Accession Q65165
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Minor capsid protein p49 (B438L) is a ~49 kDa protein. Together with the penton and the other minor capsid proteins (M1249L, p17), forms a complicated network immediately below the outer capsid shell, stabilizing the whole capsid. Plays an essential role in the formation of infectious virus particles. Especially required for the formation of the capsid vertices. During virion assembly, associates with the membrane and probably mediates the docking of the penton complex to the inner membrane, where it recruits the capsomers to form the penton core.

1. Wang, N. et al. (2019) Science (New York, N.Y.) 366, 640-644. PMID: 31624094
2. Epifano, C. et al. (2006) Journal of virology 80, 11456-66. PMID: 17005638
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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