ASFV B438L/p49 Recombinant Protein (N-His) (VK754012)

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Overview

Catalog No.	VK754012
Description	Recombinant ASFV B438L/p49 Protein, N-His (VK754012) expressed in E. coli, spanning Ser257-Ser415. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q65165
Protein length	Ser257-Ser415
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	African swine fever virus (ASFV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Minor capsid protein p49, p49, Ba71V-075, B438L
Background	Minor capsid protein p49 (B438L) is a ~49 kDa protein. Together with the penton and the other minor capsid proteins (M1249L, p17), forms a complicated network immediately below the outer capsid shell, stabilizing the whole capsid. Plays an essential role in the formation of infectious virus particles. Especially required for the formation of the capsid vertices. During virion assembly, associates with the membrane and probably mediates the docking of the penton complex to the inner membrane, where it recruits the capsomers to form the penton core. 1. Wang, N. et al. (2019) Science (New York, N.Y.) 366, 640-644. PMID: 31624094 2. Epifano, C. et al. (2006) Journal of virology 80, 11456-66. PMID: 17005638
Note	For research use only.

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SDS-PAGE for Recombinant ASFV B438L/p49 Protein, N-His

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Datasheet

ASFV B438L/p49 Recombinant Protein (N-His).pdf

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ASFV B438L/p49 Recombinant Protein (N-His) (VK754012)

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