



| Catalog No. | VK594014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Species reactivity | African swine fever virus | ||||||||
| Applications | ELISA, IHC, WB | ||||||||
| Host species | Rabbit | ||||||||
| Isotype | IgG | ||||||||
| Clonality | Polyclonal | ||||||||
| Immunogen | E. coli - derived recombinant ASFV p72/B646L (Ile423-Val641). | ||||||||
| Target | Hexon protein p72, Major capsid protein, MCP, p72, p73, Ba71V-081, B646L | ||||||||
| Purification | Purified by antigen affinity column. | ||||||||
| Accession | P22776 | ||||||||
| Form | Liquid | ||||||||
| Storage buffer | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
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| Product Usage Information |
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| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| Background | Hexon protein p72 (B646L) is a ~73 kDa protein. Capsid protein that self-assembles to form the pseudo-hexameric capsomers of the icosahedral capsid. The capsid is constructed of 2760 pseudo-hexameric capsomers and 12 pentameric capsomers, with a T=277 symmetry, about 200 nm in diameter. The capsid encapsulates the DNA-containing nucleoid, the core shell and the inner membrane. Plays an essential role in virion assembly. Involved in virus attachment to the host cell. 1. Wang, N. et al. (2019) Science (New York, N.Y.) 366, 640-644. PMID: 31624094 2. García-Escudero, R. et al. (1998) Journal of virology 72, 3185-95. PMID: 9580160 3. Gómez-Puertas, P. et al. (1996) Journal of virology 70, 5689-94. PMID: 8764090 | ||||||||
| Note | For research use only. |

Western blot analysis was performed using anti-ASFV p72/B646L polyclonal antibody at 1ug/mL on recombinant ASFV p72/B646L (Catalog No. VK594012).

Western blot analysis was performed using anti-5-P72 polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant ASFV p72/B646L (Catalog No: VK594012)
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