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Anti-ASFV p72/B646L Polyclonal Antibody (VK594014)

Anti-ASFV p72/B646L Polyclonal Antibody
Anti-ASFV p72/B646L Polyclonal Antibody
Anti-ASFV p72/B646L Polyclonal Antibody
Anti-ASFV p72/B646L Polyclonal Antibody
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Spec:
  • 50ug
  • 100ug
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Overview
Catalog No.VK594014
Species reactivityAfrican swine fever virus
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant ASFV p72/B646L (Ile423-Val641).
Target Hexon protein p72, Major capsid protein, MCP, p72, p73, Ba71V-081, B646L
Purification Purified by antigen affinity column.
Accession P22776
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Hexon protein p72 (B646L) is a ~73 kDa protein. Capsid protein that self-assembles to form the pseudo-hexameric capsomers of the icosahedral capsid. The capsid is constructed of 2760 pseudo-hexameric capsomers and 12 pentameric capsomers, with a T=277 symmetry, about 200 nm in diameter. The capsid encapsulates the DNA-containing nucleoid, the core shell and the inner membrane. Plays an essential role in virion assembly. Involved in virus attachment to the host cell.

1. Wang, N. et al. (2019) Science (New York, N.Y.) 366, 640-644. PMID: 31624094
2. García-Escudero, R. et al. (1998) Journal of virology 72, 3185-95. PMID: 9580160
3. Gómez-Puertas, P. et al. (1996) Journal of virology 70, 5689-94. PMID: 8764090
Note For research use only.
Images
  • Anti-ASFV p72/B646L Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-ASFV p72/B646L polyclonal antibody at 1ug/mL on recombinant ASFV p72/B646L (Catalog No. VK594012).

  • Anti-ASFV p72/B646L Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-5-P72 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: recombinant ASFV p72/B646L (Catalog No: VK594012)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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