ASFV p72/B646L Recombinant Protein (N-His) (VK594012)

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Catalog No.	VK594012
Description	Recombinant ASFV p72/B646L Protein, N-His (VK594012) expressed in E. coli, spanning Ile423-Val641. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P22776
Protein length	Ile423-Val641
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	African swine fever virus (ASFV)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from PBS pH7.4, 0.02%NLS, 1 mM EDTA, 4%trehalose, 1% mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Hexon protein p72, Major capsid protein (MCP), p72, p73, B646L, Ba71V-081
Background	Hexon protein p72 (B646L) is a ~73 kDa protein. Capsid protein that self-assembles to form the pseudo-hexameric capsomers of the icosahedral capsid. The capsid is constructed of 2760 pseudo-hexameric capsomers and 12 pentameric capsomers, with a T=277 symmetry, about 200 nm in diameter. The capsid encapsulates the DNA-containing nucleoid, the core shell and the inner membrane. Plays an essential role in virion assembly. Involved in virus attachment to the host cell. 1. Wang, N. et al. (2019) Science (New York, N.Y.) 366, 640-644. PMID: 31624094 2. García-Escudero, R. et al. (1998) Journal of virology 72, 3185-95. PMID: 9580160 3. Gómez-Puertas, P. et al. (1996) Journal of virology 70, 5689-94. PMID: 8764090
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant ASFV p72/B646L Protein, N-His

References

VK594012-SDS-PAGE-1.jpg

Datasheet

ASFV p72/B646L Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

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ASFV p72/B646L Recombinant Protein (N-His) (VK594012)

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