Anti-ASIC1 Recombinant Antibody (N271/28) (HX873023)

Name: Anti-ASIC1 Recombinant Antibody (N271/28)
Brand: abinScience
SKU: HX873023
Price: 196 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HX873023

Description

Anti-ASIC1 Recombinant Antibody (N271/28) (HX873023) is a mouse monoclonal antibody detecting ASIC1 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N271/28

Target

ASIC1, Amiloride-sensitive cation channel 2, neuronal, BNaC2, Brain sodium channel 2, ACCN2, Acid-sensing ion channel 1, BNAC2

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-ASIC1 Antibody (N271/28) (abinScience Cat# HX873023, RRID:AB_3728304)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Acid-sensing ion channel 1 (ASIC1) is a ~59 kDa protein. Forms voltage-independent, pH-gated trimeric sodium channels that act as postsynaptic excitatory receptors in the nervous system, playing a crucial role in regulating synaptic plasticity, learning, and memory. Upon extracellular pH drop this channel elicits transient, fast activating, and completely desensitizing inward currents. Displays high selectivity for sodium ions but can also permit the permeation of other cations. Regulates more or less directly intracellular calcium concentration and CaMKII phosphorylation, and thereby the density of dendritic spines. Modulates neuronal activity in the circuits underlying innate fear.

1. Hoagland, EN. et al. (2010) The Journal of biological chemistry 285, 41852-62. PMID: 21036899

2. Sun, D. et al. (2020) eLife 9. PMID: 32915133

3. Wu, Y. et al. (2021) eLife 10. PMID: 34319232

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-ASIC1 Antibody (N271/28)

References

Datasheet

Anti-ASIC1 Recombinant Antibody (N271/28).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-ASIC1 Recombinant Antibody (N271/28) (HX873023)

Overview

Images

References

Datasheet

SDS