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Anti-Aspergillus flavus Uricase Polyclonal Antibody (JA150014)

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Overview
Catalog No.JA150014
Description
Anti-Aspergillus flavus Uricase Polyclonal Antibody (JA150014) is a rabbit polyclonal antibody detecting Uricase. Suitable for Aspergillus flavus.
Species reactivityAspergillus flavus
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Aspergillus flavus Uricase (Met1-Leu302).
Target EC:1.7.3.3, Rasburicase, Uox, Urate oxidase, Uricase, uaZ
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession Q00511
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Uricase is a ~34 kDa protein. Urate oxidase is a cofactorless enzyme involved in the metabolism of purines (PubMed:1339455, PubMed:17020862, PubMed:28272834, Ref.3). Catalyzes, in the presence of molecular oxygen, the hydroxylation of uric acid to metastable 5-hydroxyisourate (5-HIU) which is further degraded to allantoin.

1. Legoux, R. et al. (1992) The Journal of biological chemistry 267, 8565-70. PMID: 1339455
2. Bayol, A. et al. (1995) Biophysical chemistry 54, 229-35. PMID: 17020862
3. Cheuk, DK. et al. (2017) The Cochrane database of systematic reviews 3, CD006945. PMID: 28272834
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₂ (Working Conc.)
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Anti-Aspergillus flavus Uricase Polyclonal Antibody

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