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Anti-Aspergillus flavus Uricase/Uox Polyclonal Antibody (JA150014)

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Overview
Catalog No.JA150014
Description
Anti-Aspergillus flavus Uricase Polyclonal Antibody (JA150014) is a rabbit polyclonal antibody detecting Uricase. Suitable for Aspergillus flavus.
Species reactivityAspergillus flavus
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Aspergillus flavus Uricase (Met1-Leu302).
Target EC:1.7.3.3, Rasburicase, Uox, Urate oxidase, Uricase, uaZ
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Purified by antigen affinity column.
Accession Q00511
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Background

Uricase is a ~34 kDa protein. Urate oxidase is a cofactorless enzyme involved in the metabolism of purines (PubMed:1339455, PubMed:17020862, PubMed:28272834, Ref.3). Catalyzes, in the presence of molecular oxygen, the hydroxylation of uric acid to metastable 5-hydroxyisourate (5-HIU) which is further degraded to allantoin.

1. Legoux, R. et al. (1992) The Journal of biological chemistry 267, 8565-70. PMID: 1339455
2. Bayol, A. et al. (1995) Biophysical chemistry 54, 229-35. PMID: 17020862
3. Cheuk, DK. et al. (2017) The Cochrane database of systematic reviews 3, CD006945. PMID: 28272834
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Aspergillus flavus Uricase/Uox Polyclonal Antibody

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