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Aspergillus flavus Uricase Recombinant Protein (N-His) (JA150012)

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Overview
Catalog No.JA150012
Description
Recombinant Aspergillus flavus Uricase Protein, N-His (JA150012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
  • E. coli Expression — High-yield, cost-effective production.
  • High Purity — >90% as determined by SDS-PAGE.
Expression systemE. coli
AccessionQ00511
Protein lengthMet1-Leu302
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
SpeciesAspergillus flavus
Nature Recombinant
Endotoxin level Please contact with the lab for this information.
Purity >90% as determined by SDS-PAGE.
Form Lyophilized
Storage buffer Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate NamesEC:1.7.3.3, Rasburicase, Uox, Urate oxidase, Uricase, uaZ
Background

Uricase is a ~34 kDa protein. Urate oxidase is a cofactorless enzyme involved in the metabolism of purines (PubMed:1339455, PubMed:17020862, PubMed:28272834, Ref.3). Catalyzes, in the presence of molecular oxygen, the hydroxylation of uric acid to metastable 5-hydroxyisourate (5-HIU) which is further degraded to allantoin.

1. Legoux, R. et al. (1992) The Journal of biological chemistry 267, 8565-70. PMID: 1339455
2. Bayol, A. et al. (1995) Biophysical chemistry 54, 229-35. PMID: 17020862
3. Cheuk, DK. et al. (2017) The Cochrane database of systematic reviews 3, CD006945. PMID: 28272834
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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