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Anti-BAF53A Recombinant Antibody (N336B/26) (HF579013)

Anti-BAF53A Recombinant Antibody (N336B/26)
Anti-BAF53A Recombinant Antibody (N336B/26)
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Overview
Catalog No.HF579013
Description
Anti-BAF53a Recombinant Antibody (N336B/26) (HF579013) is a mouse monoclonal antibody detecting ACTL6A/BAF53a in IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
ApplicationsIHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDN336B/26
Target BAF53, BAF53A, INO80K, BRG1-associated factor 53A, ArpNbeta, Actin-related protein Baf53a, INO80 complex subunit K, ACTL6A, Actin-like protein 6A, 53 kDa BRG1-associated factor A
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
RRID

Anti-BAF53a Antibody (N336B/26) (abinScience Cat# HF579013, RRID:AB_3728086)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Actin-like protein 6A (ACTL6A/BAF53a) is a ~47 kDa protein. Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Required for maximal ATPase activity of SMARCA4/BRG1/BAF190A and for association of the SMARCA4/BRG1/BAF190A containing remodeling complex BAF with chromatin/nuclear matrix. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state.

Note For research use only.
Images
  • Anti-BAF53A Recombinant Antibody (N336B/26)

    SDS-PAGE

    SDS-PAGE for Anti-BAF53a Antibody (N336B/26)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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