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Anti-BBS5 Recombinant Antibody (N304B/99) (HA919033)

Anti-BBS5 Recombinant Antibody (N304B/99)
Anti-BBS5 Recombinant Antibody (N304B/99)
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  • 50ug
  • 100ug
  • 1mg
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Overview
Catalog No.HA919033
Description
Anti-BBS5 Recombinant Antibody (N304B/99) (HA919033) is a mouse monoclonal antibody detecting BBS5 in IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
ApplicationsIHC, WB
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDN304B/99
Target Bardet-Biedl syndrome 5 protein, BBS5
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
RRID

Anti-BBS5 Antibody (N304B/99) (abinScience Cat# HA919033, RRID:AB_3728560)
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Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

BBSome complex member BBS5 is a ~38 kDa protein. The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.

Note For research use only.
Images
  • Anti-BBS5 Recombinant Antibody (N304B/99)

    SDS-PAGE

    SDS-PAGE for Anti-BBS5 Antibody (N304B/99)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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