Anti-beta-2 Adrenergic R/ADRB2 Recombinant Antibody (N430/26) (HY357053)

Name: Anti-beta-2 Adrenergic R/ADRB2 Recombinant Antibody (N430/26)
Brand: abinScience
SKU: HY357053
Price: 196 USD
Availability: InStock

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1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY357053

Description

Anti-beta-2 Adrenergic R/ADRB2 Recombinant Antibody (N430/26) (HY357053) is a mouse monoclonal antibody detecting beta-2 Adrenergic R/ADRB2 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N430/26

Target

Beta-2 adrenergic receptor, ADRB2R, Beta-2 adrenoreceptor, ADRB2, B2AR, Beta-2 adrenoceptor

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-Beta-2 adrenergic receptor Antibody (N430/26) (abinScience Cat# HY357053, RRID:AB_3728101)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Beta-2 adrenergic receptor is a ~46 kDa protein. G protein-coupled receptor for catecholamines that couples to both G(s) and G(i) proteins, activating bifurcated signaling pathways. ADRB2 binds epinephrine (Epi) with an approximately 30-fold greater affinity than norepinephrine (NE). In the heart, Epi- and NE-activated ADRB2 induces rapid and slow cardiomyocyte contraction rate, respectively. Both NE and Epi promote coupling to G(s)/PKA pathway to regulate myocyte contraction rate. Epi also promotes ADRB2 coupling to G(i) proteins to exert cardioprotective effects especially in the conditions of hypoxia and oxidative stress through the G(i)/PI3K/Akt signaling pathway.

1. Zhu, WZ. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 1607-12. PMID: 11171998

2. Wang, Y. et al. (2008) The Journal of biological chemistry 283, 1799-807. PMID: 18056263

3. Allen, JM. et al. (1988) The EMBO journal 7, 133-8. PMID: 2834198

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Beta-2 adrenergic receptor Antibody (N430/26)

References

Datasheet

Anti-beta-2 Adrenergic R/ADRB2 Recombinant Antibody (N430/26).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-beta-2 Adrenergic R/ADRB2 Recombinant Antibody (N430/26) (HY357053)

Overview

Images

References

Datasheet

SDS