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Anti-Biotin Recombinant Antibody (M33) (YP709013)

Anti-Biotin Recombinant Antibody (M33)
Anti-Biotin Recombinant Antibody (M33)
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Overview
Catalog No.YP709013
Species reactivityGeneral
ApplicationsELISA, SPR, WB
Host speciesMouse
IsotypeIgG2a
Clone IDM33
Clonality Monoclonal
Target Biotin, Vitamin B7, Vitamin H, BTN
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
References 1. Zempleni, et al. Biotin biochemistry and human requirements. J Nutr Biochem, 1999. 10(3): p. 128-38. [YP709013]
2. Mock, D.M.. Biotin status: which are valid indicators and how do we know? J Nutr, 1999. 129(2S Suppl): p. 498S-503S. [YP709013]
3. Zempleni J, et al. Biotin. Biofactors. 2009 Jan-Feb;35(1):36-46. [YP709013]
4. Vadlapudi AD, et al. Biotin uptake by T47D breast cancer cells: functional and molecular evidence of sodium-dependent multivitamin transporter (SMVT). Int J Pharm. 2013 Jan 30;441(1-2):535-43. [YP709013]
5. Sghaier R, et al. Biotin attenuation of oxidative stress, mitochondrial dysfunction, lipid metabolism alteration and 7β-hydroxycholesterol-induced cell death in 158N murine oligodendrocytes. Free Radic Res. 2019 May;53(5):535-561. [YP709013]
6. Aldahmash BA, et al. Biotin amelioration of nephrotoxicity in streptozotocin-induced diabetic mice. Saudi J Biol Sci. 2015 Sep;22(5):564-9. [YP709013]
7. He P, et al. Dietary biotin deficiency decreased growth performance and impaired the immune function of the head kidney, spleen and skin in on-growing grass carp (Ctenopharyngodon idella). Fish Shellfish Immunol. 2020 Feb;97:216-234. View More [YP709013]
Note For research use only.
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  • Anti-Biotin Recombinant Antibody (M33)

    SDS-PAGE

    SDS-PAGE for Anti-Biotin Antibody (M33).

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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