Anti-Bordetella pertussis ptxA/PTXS1 Polyclonal Antibody (JN968014)

Name: Anti-Bordetella pertussis ptxA/PTXS1 Polyclonal Antibody
Brand: abinScience
SKU: JN968014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

JN968014

Species reactivity

Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Bordetella pertussis ptxA/PTXS (Asp35-Phe269).

Target

Pertussis toxin subunit 1, PTX S1, Islet-activating protein S1, IAP S1, NAD-dependent ADP-ribosyltransferase, 2.4.2.-, ptxA, BP3783

Purification

Purified by antigen affinity column.

Accession

P04977

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Pertussis toxin subunit 1 (ptxA/PTXS1) is a ~29 kDa protein. S1 is an NAD-dependent ADP-ribosyltransferase, which plays a crucial role in the pathogenesis of B.pertussis causing disruption of normal host cellular regulation. It catalyzes the ADP-ribosylation of a cysteine in the alpha subunit of host heterotrimeric G proteins. In the absence of G proteins it also catalyzes the cleavage of NAD(+) into ADP-ribose and nicotinamide. It irreversibly uncouples the G-alpha GTP-binding proteins from their membrane receptors.

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Bordetella pertussis ptxA/PTXS1 polyclonal antibody at 1ug/mL on recombinant Bordetella pertussis ptxA/PTXS1 (Catalog No. JN968012).
Western blot

Western blot analysis was performed using anti-PT polyclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant Bordetella pertussis ptxA/PTXS1 (Catalog No: JN968012)