Anti-Botch Recombinant Antibody (N116/10) (HP690013)

Name: Anti-Botch Recombinant Antibody (N116/10)
Brand: abinScience
SKU: HP690013
Price: 196 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP690013

Description

Anti-Botch Recombinant Antibody (N116/10) (HP690013) is a mouse monoclonal antibody detecting CHAC1/Botch in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N116/10

Target

Glutathione-specific gamma-glutamylcyclotransferase 1, Gamma-GCG 1, Cation transport regulator-like protein 1, CHAC1, Botch, BOTCH, Blocks Notch protein

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-Botch Antibody (N116/10) (abinScience Cat# HP690013, RRID:AB_3728707)
_x005F_x000D_

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Glutathione-specific gamma-glutamylcyclotransferase 1 (CHAC1/Botch) is a ~24 kDa protein. Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos.

1. Kaur, A. et al. (2017) The Journal of biological chemistry 292, 638-651. PMID: 27913623

2. Mungrue, IN. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 466-76. PMID: 19109178

3. Chi, Z. et al. (2012) Developmental cell 22, 707-20. PMID: 22445366

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Botch Antibody (N116/10)

References

Datasheet

Anti-Botch Recombinant Antibody (N116/10).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Botch Recombinant Antibody (N116/10) (HP690013)

Overview

Images

References

Datasheet

SDS