Anti-Calreticulin/CALR Polyclonal Antibody (HB190014)

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Overview

Catalog No.

HB190014

Description

Anti-Calreticulin/CALR Polyclonal Antibody (HB190014) is a rabbit polyclonal antibody detecting Calreticulin in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Cricetulus griseus.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat, Bos taurus, Chlorocebus aethiops, Cricetulus griseus, Macaca fascicularis, Macaca fuscata fuscata, Oryctolagus cuniculus, Pig

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human Calreticulin/CALR (Glu18-Pro204).

Target

Calreticulin, ERp60, CRTC, Calregulin, grp60, HACBP, Endoplasmic reticulum resident protein 60, CALR, CRP55

Purification

Purified by antigen affinity column.

Accession

P27797

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Calreticulin is a ~48 kDa protein. Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.

1. Nauseef, WM. et al. (1995) The Journal of biological chemistry 270, 4741-7. PMID: 7876246

2. Holaska, JM. et al. (2001) The Journal of cell biology 152, 127-40. PMID: 11149926

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CALR polyclonal antibody at 1ug/mL on various samples.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
Lane 3: HL-60 cell lysate

References

Datasheet

Anti-Calreticulin/CALR Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Calreticulin/CALR Polyclonal Antibody (HB190014)

Overview

Images

References

Datasheet

SDS

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