Please ensure Javascript is enabled for purposes of website accessibility
Home > Products > Antibodies > Primary antibodies

Anti-cAMP Monoclonal Antibody (1A187) (YP051025)

Anti-cAMP Monoclonal Antibody (1A187)
Anti-cAMP Monoclonal Antibody (1A187)
Price(USD): --
Spec:
  • 50ug
  • 100ug
Number:
Get quote
  • Overview

  • Images

  • References

  • Datasheet

  • SDS

Overview
Catalog No.YP051025
Description
Anti-cAMP Monoclonal Antibody (1A187) [1A187] (YP051025) is a mouse monoclonal antibody detecting cAMP in ELISA. Suitable for General.
Species reactivityGeneral
ApplicationsELISA
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG1
Clone ID1A187
Immunogen cAMP
Target cAMP, Cyclic Adenosine Monophosphate
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Adenosine 3', 5'-cyclic monophosphate (cAMP) is a key second messenger molecule in intracellular signal transduction. Monitoring cAMP levels is one of the most common ways to screen for agonists and antagonists of G protein–coupled receptors(GPCRs). Bioyeargene cAMP ELISA Kit is based on the competition between HRP-labeled cAMP and free cAMP for fixed amounts of mouse monoclonal anti-cAMP antibody(cAMP MAb) binding sites. Because the concentration of the HRP-labeled cAMP is held constant while the concentration of cAMP varies, the amount of HRP-labeled cAMP that is able to bind to the cAMP MAb will be inversely proportional to the concentration of cAMP in the well. This antigen-antibody complex binds to the goat anti-mouse IgG that has been previously attached to the well. The plate is washed to remove any unbound reagents and then developed. The ELISA typically displays an IC50(50% B/B0) of approximately 12 pmol/mL and a detection limit of approximately 0.15 pmol/mL.

Note For research use only.
Images
  • Anti-cAMP Monoclonal Antibody (1A187)

    SDS-PAGE

    SDS-PAGE for Anti-cAMP Monoclonal Antibody (1A187)

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommendation

Contact us for custom quotes, bulk requests and any other issues.

Mail: [email protected]

Distributor list