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Anti-cas13a Polyclonal Antibody (JN124014)

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Overview
Catalog No.JN124014
Description
Anti-cas13a Polyclonal Antibody (JN124014) is a rabbit polyclonal antibody detecting CRISPR-associated endoribonuclease Cas13a; EndoRNase; 3.1.-.-; LbaCas13a; cas13a in ELISA, IHC, WB.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityLachnospiraceae bacterium
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant cas13a (Val17-Asn319).
Target CRISPR-associated endoribonuclease Cas13a; EndoRNase; 3.1.-.-; LbaCas13a; cas13a
Endotoxin level Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Accession P0DPB7
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

CRISPR-associated endoribonuclease Cas13a is a ~168 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. Unlike many single-component effectors, this CRISPR-Cas system targets RNA. CRISPR clusters are transcribed from pre-CRISPR RNA (crRNA) and processed into crRNA by this protein. Cleaves linear target ssRNA in a pre-crRNA-dependent fashion, preferentially around A residues.

1. East-Seletsky, A. et al. (2017) Molecular cell 66, 373-383.e3. PMID: 28475872
Note For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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Anti-cas13a Polyclonal Antibody

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