cas13a Recombinant Protein (N-His) (JN124012)

Name: cas13a Recombinant Protein (N-His)
Brand: abinScience
SKU: JN124012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	JN124012
Description	Recombinant cas13a Protein, N-His (JN124012) expressed in E. coli, spanning Val17-Asn319. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P0DPB7
Protein length	Val17-Asn319
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Lachnospiraceae bacterium
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	37.41 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CRISPR-associated endoribonuclease Cas13a; EndoRNase; 3.1.-.-; LbaCas13a; cas13a
Background	CRISPR-associated endoribonuclease Cas13a is a ~168 kDa protein. CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. Unlike many single-component effectors, this CRISPR-Cas system targets RNA. CRISPR clusters are transcribed from pre-CRISPR RNA (crRNA) and processed into crRNA by this protein. Cleaves linear target ssRNA in a pre-crRNA-dependent fashion, preferentially around A residues. 1. East-Seletsky, A. et al. (2017) Molecular cell 66, 373-383.e3. PMID: 28475872
Note	For research use only

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SDS-PAGE for Recombinant cas13a Protein, N-His

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Datasheet

cas13a Recombinant Protein (N-His).pdf

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cas13a Recombinant Protein (N-His) (JN124012)

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