Anti-CASP6 Polyclonal Antibody (HX844014)

Name: Anti-CASP6 Polyclonal Antibody
Brand: abinScience
SKU: HX844014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HX844014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human CASP6 (Ala194-Asn293).

Target

Apoptotic protease Mch-2, Caspase-6, CASP-6, MCH2, CASP6

Purification

Purified by antigen affinity column.

Accession

P55212

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Caspase-6 (CASP6) is a ~33 kDa protein. Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity. Acts as a non-canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation. Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution. Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis. Cleaves PARK7/DJ-1 in cells undergoing apoptosis.

1. Klaiman, G. et al. (2009) Biochimica et biophysica acta 1793, 592-601. PMID: 19133298

2. van Raam, BJ. et al. (2013) Cell death and differentiation 20, 86-96. PMID: 22858542

3. Hill, ME. et al. (2016) ACS chemical biology 11, 1603-12. PMID: 27032039

4. Dagbay, KB. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E7977-E7986. PMID: 28864531

5. MacPherson, DJ. et al. (2019) The Journal of biological chemistry 294, 71-88. PMID: 30420425

6. Zheng, M. et al. (2020) Cell 181, 674-687.e13. PMID: 32298652

7. Orth, K. et al. (1996) The Journal of biological chemistry 271, 16443-6. PMID: 8663580

8. Ruchaud, S. et al. (2002) The EMBO journal 21, 1967-77. PMID: 11953316

9. Lin, HH. et al. (2007) Journal of biomedical science 14, 681-94. PMID: 17401638

Note

For research use only.

Images

References

Datasheet

Anti-CASP6 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-CASP6 Polyclonal Antibody (HX844014)

Overview

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Datasheet

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