ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
13.58 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Caspase-6 (CASP6) is a ~33 kDa protein. Cysteine protease that plays essential roles in programmed cell death, axonal degeneration, development and innate immunity. Acts as a non-canonical executioner caspase during apoptosis: localizes in the nucleus and cleaves the nuclear structural protein NUMA1 and lamin A/LMNA thereby inducing nuclear shrinkage and fragmentation. Lamin-A/LMNA cleavage is required for chromatin condensation and nuclear disassembly during apoptotic execution. Acts as a regulator of liver damage by promoting hepatocyte apoptosis: in absence of phosphorylation by AMP-activated protein kinase (AMPK), catalyzes cleavage of BID, leading to cytochrome c release, thereby participating in nonalcoholic steatohepatitis. Cleaves PARK7/DJ-1 in cells undergoing apoptosis.
1. Klaiman, G. et al. (2009) Biochimica et biophysica acta 1793, 592-601. PMID: 19133298
2. van Raam, BJ. et al. (2013) Cell death and differentiation 20, 86-96. PMID: 22858542
3. Hill, ME. et al. (2016) ACS chemical biology 11, 1603-12. PMID: 27032039
4. Dagbay, KB. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E7977-E7986. PMID: 28864531
5. MacPherson, DJ. et al. (2019) The Journal of biological chemistry 294, 71-88. PMID: 30420425
6. Zheng, M. et al. (2020) Cell 181, 674-687.e13. PMID: 32298652
7. Orth, K. et al. (1996) The Journal of biological chemistry 271, 16443-6. PMID: 8663580
8. Ruchaud, S. et al. (2002) The EMBO journal 21, 1967-77. PMID: 11953316
9. Lin, HH. et al. (2007) Journal of biomedical science 14, 681-94. PMID: 17401638
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human CASP6 Protein, N-His
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