Anti-CBX3 Polyclonal Antibody (HC291014)

Name: Anti-CBX3 Polyclonal Antibody
Brand: abinScience
SKU: HC291014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC291014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CBX3 (Met1-Gln183).

Target

HECH, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, Chromobox protein homolog 3, HP1 gamma, CBX3

Purification

Purified by antigen affinity column.

Accession

Q13185

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Chromobox protein homolog 3 (CBX3) is a ~20 kDa protein. Component of heterochromatin, which recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Also recognizes and binds histone H1.4 methylated at 'Lys-26' (H1.4K26me). Excluded from chromatin when histone H1.4 is Simultaneously methylated at Lys-26 (H1.4K26me) and phosphorylated at Ser-27 (H1.4S27Ph). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.

1. Lachner, M. et al. (2001) Nature 410, 116-20. PMID: 11242053

2. Daujat, S. et al. (2005) The Journal of biological chemistry 280, 38090-5. PMID: 16127177

3. Obuse, C. et al. (2004) Nature cell biology 6, 1135-41. PMID: 15502821

4. Bot, C. et al. (2017) Journal of cell science 130, 1134-1146. PMID: 28167679

Note

For research use only.

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References

Datasheet