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Anti-CCL4/MIP-1-beta Polyclonal Antibody (HY327014)

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Overview
Catalog No.HY327014
Description
Anti-CCL4/MIP-1-beta Polyclonal Antibody (HY327014) is a rabbit polyclonal antibody detecting MIP-1-beta in ELISA, IHC, WB. Suitable for Human and Macaca mulatta.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human CCL4/MIP-1-beta (Ala24-Asn92).
Target MIP-1-beta(1-69), MIP-1-beta, C-C motif chemokine 4, Protein H400, ACT-2, HC21, T-cell activation protein 2, Lymphocyte activation gene 1 protein, LAG-1, SIS-gamma, Small-inducible cytokine A4, G-26 T-lymphocyte-secreted protein, Macrophage inflammatory protein 1-beta, LAG1, PAT 744, CCL4, SCYA4, MIP1B
Purification Purified by antigen affinity column.
Accession P13236
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

C-C motif chemokine 4 (CCL4/MIP-1-beta) is a ~10 kDa protein. Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells.

Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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