Anti-CD144/CDH5 Polyclonal Antibody (HW589014)

Name: Anti-CD144/CDH5 Polyclonal Antibody
Brand: abinScience
SKU: HW589014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HW589014

Description

Anti-CD144/CDH5 Polyclonal Antibody (HW589014) is a rabbit polyclonal antibody detecting CD144 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD144/CDH5 (Val68-Thr307).

Target

7B4 antigen, CD144, VE-cadherin, Cadherin-5, CDH5, Vascular endothelial cadherin

Purification

Purified by antigen affinity column.

Accession

P33151

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Cadherin-5 (CD144/CDH5) is a ~87 kDa protein. Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play a important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Plays a role in coupling actin fibers to cell junctions in endothelial cells, via acting as a cell junctional complex anchor for AMOTL2 and MAGI1.

1. Brasch, J. et al. (2011) Journal of molecular biology 408, 57-73. PMID: 21269602

2. Shimoyama, Y. et al. (2000) The Biochemical journal 349, 159-67. PMID: 10861224

3. Lampugnani, MG. et al. (2010) Journal of cell science 123, 1073-80. PMID: 20332120

4. Abiko, H. et al. (2015) Journal of cell science 128, 1683-95. PMID: 25795300

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD144/CDH5 polyclonal antibody at 1ug/mL on recombinant CD144/CDH5 (Catalog No. HW589012).

References

Datasheet