Anti-CD150/SLAMF1 Polyclonal Antibody (HC275014)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HC275014

Description

Anti-CD150/SLAMF1 Polyclonal Antibody (HC275014) is a rabbit polyclonal antibody detecting SLAMF1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD150/SLAMF1 (Phe19-Lys236).

Target

SLAM family member 1, IPO-3, CDw150, SLAMF1, SLAM, CD150, Signaling lymphocytic activation molecule

Purification

Purified by antigen affinity column.

Accession

Q13291

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Signaling lymphocytic activation molecule (CD150/SLAMF1) is a ~37 kDa protein. Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. SLAMF1-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAMF1 signaling seem to exist: one depending on SH2D1A (and perhaps SH2D1B) and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

1. Howie, D. et al. (2002) Blood 99, 957-65. PMID: 11806999

2. Li, C. et al. (2003) The Journal of biological chemistry 278, 3852-9. PMID: 12458214

3. Réthi, B. et al. (2006) Blood 107, 2821-9. PMID: 16317102

4. Yurchenko, MY. et al. (2010) Immunology and cell biology 88, 565-74. PMID: 20231852

5. Berger, SB. et al. (2010) Nature immunology 11, 920-7. PMID: 20818396

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD150/SLAMF1 polyclonal antibody at 1ug/mL on recombinant CD150/SLAMF1 (Catalog No. HC275012).

References

Datasheet