Anti-CD156b/ADAM17 Polyclonal Antibody (HX200014)

Name: Anti-CD156b/ADAM17 Polyclonal Antibody
Brand: abinScience
SKU: HX200014
Price: 168 USD
Availability: InStock

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Spec:

50ug
100ug

Number:

Overview
Images
References
Datasheet

Overview

Catalog No.

HX200014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD156b/ADAM17 (Lys226-Arg473).

Target

TACE, Snake venom-like protease, ADAM17, CD156b, CSVP, ADAM 17, Disintegrin and metalloproteinase domain-containing protein 17, TNF-alpha convertase, TNF-alpha-converting enzyme

Purification

Purified by antigen affinity column.

Accession

P78536

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Disintegrin and metalloproteinase domain-containing protein 17 (CD156b/ADAM17) is a ~93 kDa protein. Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins including adhesion proteins, growth factor precursors and cytokines important for inflammation and immunity. Cleaves the membrane-bound precursor of TNF to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT).

1. Boskovski, MT. et al. (2013) Nature 504, 456-9. PMID: 24226769

2. Heurich, A. et al. (2014) Journal of virology 88, 1293-307. PMID: 24227843

3. Riethmueller, S. et al. (2017) PLoS biology 15, e2000080. PMID: 28060820

4. Feuerbach, D. et al. (2017) Neuroscience letters 660, 109-114. PMID: 28923481

5. Müller, M. et al. (2022) Cells 11. PMID: 36078095

6. Moss, ML. et al. (1997) Nature 385, 733-6. PMID: 9034191

7. Rabquer, BJ. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 1777-85. PMID: 20592283

8. Thathiah, A. et al. (2003) The Journal of biological chemistry 278, 3386-94. PMID: 12441351

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD156b/ADAM17 polyclonal antibody at 1ug/mL on recombinant CD156b/ADAM17 (Catalog No. HX200012).

References

Datasheet