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Anti-CD29/ITGB1 Antibody (AIIB2) (HY248307)

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Overview
Catalog No.HY248307
Description
Anti-CD29/ITGB1 Antibody (AIIB2) [AIIB2] (HY248307) is a mouse monoclonal antibody detecting ITGB1 in ELISA, FCM, FuncS, ICC, IF, IHC, IP, WB. Suitable for Human.
Highlights
  • Multi-Application — Validated across multiple applications.
  • Flow Cytometry — Validated for flow cytometry applications.
Species reactivityHuman
ApplicationsELISA, FCM, FuncS, ICC, IF, IHC, IP, WB
Host speciesMouse
IsotypeIgG2a
Clone IDAIIB2
Clonality Monoclonal
Target CD29, FNRB, Fibronectin receptor subunit beta, GPIIA, Glycoprotein IIa, ITGB1, Integrin beta-1, MDF2, MSK12, VLA-4 subunit beta
Endotoxin level <10 EU/mg
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P05556
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Integrin beta-1 (CD29/ITGB1) is a ~88 kDa protein. Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen.

1. Fujita, M. et al. (2012) Journal of immunology (Baltimore, Md. : 1950) 189, 5809-19. PMID: 23125415
2. Fujita, M. et al. (2014) PloS one 9, e96372. PMID: 24789099
3. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536
4. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877
5. Bax, DV. et al. (2003) The Journal of biological chemistry 278, 34605-16. PMID: 12807887
6. Jovanovic, J. et al. (2007) The Journal of biological chemistry 282, 6743-51. PMID: 17158881
7. Schumacher, S. et al. (2021) Science advances 7. PMID: 33962943
8. Takada, YK. et al. (2017) The Journal of biological chemistry 292, 20067-20075. PMID: 29030430
9. Takada, YK. et al. (2019) Journal of immunology (Baltimore, Md. : 1950) 203, 1383-1391. PMID: 31331973
10. Morris, GE. et al. (2022) British journal of pharmacology 179, 4958-4973. PMID: 35802072
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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