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Anti-CD322/JAM2 Polyclonal Antibody (HX993014)

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Overview
Catalog No.HX993014
Description
Anti-CD322/JAM2 Polyclonal Antibody (HX993014) is a rabbit polyclonal antibody detecting CD322 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant Human CD322/JAM2 (Tyr74-Val250).
Target JAM-B, VEJAM, Junctional adhesion molecule B, JAM2, VE-JAM, JAM-2, C21orf43, Vascular endothelial junction-associated molecule, Junctional adhesion molecule 2, CD322
Purification Purified by antigen affinity column.
Accession P57087
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Junctional adhesion molecule B (CD322/JAM2) is a ~33 kDa protein. Junctional adhesion protein that mediates heterotypic cell-cell interactions with its cognate receptor JAM3 to regulate different cellular processes. Plays a role in homing and mobilization of hematopoietic stem and progenitor cells within the bone marrow. At the surface of bone marrow stromal cells, it contributes to the retention of the hematopoietic stem and progenitor cells expressing JAM3. Plays a central role in leukocytes extravasation by facilitating not only transmigration but also tethering and rolling of leukocytes along the endothelium. Tethering and rolling of leukocytes are dependent on the binding by JAM2 of the integrin alpha-4/beta-1.

1. Arrate, MP. et al. (2001) The Journal of biological chemistry 276, 45826-32. PMID: 11590146
2. Liang, TW. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 1618-26. PMID: 11823489
3. Arcangeli, ML. et al. (2014) Stem cells (Dayton, Ohio) 32, 1043-54. PMID: 24357068
4. Johnson-Léger, CA. et al. (2002) Blood 100, 2479-86. PMID: 12239159
5. Cunningham, SA. et al. (2002) The Journal of biological chemistry 277, 27589-92. PMID: 12070135
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-CD322/JAM2 Polyclonal Antibody

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