Anti-CHD4 Polyclonal Antibody (HC562014)

Name: Anti-CHD4 Polyclonal Antibody
Brand: abinScience
SKU: HC562014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HC562014

Description

Anti-CHD4 Polyclonal Antibody (HC562014) is a rabbit polyclonal antibody detecting CHD4 in ELISA, IHC, WB. Suitable for Human and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CHD4 (Thr1706-Gln1912).

Target

ATP-dependent helicase CHD4, CHD-4, CHD4, Chromodomain-helicase-DNA-binding protein 4, EC:3.6.4.-, Mi-2 autoantigen 218 kDa protein, Mi2-beta

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q14839

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternate Names

Q14839

Background

ATP-dependent chromatin remodeler CHD4 is a ~218 kDa protein. ATP-dependent chromatin-remodeling factor that binds and distorts nucleosomal DNA. Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin. Localizes to acetylated damaged chromatin in a ZMYND8-dependent manner, to promote transcriptional repression and double-strand break repair by homologous recombination. Involved in neurogenesis.

1. Hoffmeister, H. et al. (2017) Nucleic acids research 45, 10534-10554. PMID: 28977666

2. Farnung, L. et al. (2020) eLife 9. PMID: 32543371

3. Le Guezennec, X. et al. (2006) Molecular and cellular biology 26, 843-51. PMID: 16428440

4. Sillibourne, JE. et al. (2007) Molecular biology of the cell 18, 3667-80. PMID: 17626165

5. Tong, JK. et al. (1998) Nature 395, 917-21. PMID: 9804427

6. Gong, F. et al. (2015) Genes & development 29, 197-211. PMID: 25593309

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-CHD4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

References

Datasheet

Anti-CHD4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-CHD4 Polyclonal Antibody (HC562014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us