Anti-CSPG4 Polyclonal Antibody (HV349014)

Name: Anti-CSPG4 Polyclonal Antibody
Brand: abinScience
SKU: HV349014
Price: 96 USD
Availability: InStock

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Spec:

20ug
50ug
100ug

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV349014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CSPG4 (Ser1705-Ile1942).

Target

Chondroitin sulfate proteoglycan 4, CSPG4, Chondroitin sulfate proteoglycan NG2, MCSP, Melanoma chondroitin sulfate proteoglycan, Melanoma-associated chondroitin sulfate proteoglycan

Purification

Purified by antigen affinity column.

Accession

Q6UVK1

RRID

Anti-CSPG4 Polyclonal Antibody (abinScience Cat# HV349014, RRID:AB_3723050)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Chondroitin sulfate proteoglycan 4 (CSPG4/HMW-MAA) is a ~250 kDa protein. Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties.

Note

For research use only.

Images

Immunofluorescence

U2OS cells were fixed with 4% paraformaldehyde for 20 minutes, followed by blocking with 5% goat serum for 1 hour. The cells were then incubated with a primary antibody targeting CSPG4 (HV349014) at a concentration of 28 ug/ml overnight at 4°C. Afterward, the cells were incubated with a secondary antibody, Goat Anti-Rabbit IgG (Alexa Fluor-488) for 50 minutes at room temperature. The localization of CSPG4 was visualized (shown in green), and nuclear DNA was stained with DAPI (shown in blue).

References

Datasheet