Anti-Dicer Recombinant Antibody (N167/12) (HV034033)

Name: Anti-Dicer Recombinant Antibody (N167/12)
Brand: abinScience
SKU: HV034033
Price: 196 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HV034033

Description

Anti-Dicer Recombinant Antibody (N167/12) (HV034033) is a mouse monoclonal antibody detecting DICER1 in IHC, WB.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Applications

IHC, WB

Host species

Mouse

Clonality

Monoclonal

Isotype

IgG2a, kappa

Clone ID

N167/12

Target

DICER, DICER1, Helicase MOI, Endoribonuclease Dicer, Helicase with RNase motif, HERNA, KIAA0928

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

RRID

Anti-Dicer Antibody (N167/12) (abinScience Cat# HV034033, RRID:AB_3728902)
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Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Endoribonuclease Dicer (DICER1) is a ~218 kDa protein. Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Dicer Antibody (N167/12)

References

Datasheet

Anti-Dicer Recombinant Antibody (N167/12).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Dicer Recombinant Antibody (N167/12) (HV034033)

Overview

Images

References

Datasheet

SDS