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Anti-E Protein Polyclonal Antibody (VK672014)

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Overview
Catalog No.VK672014
Description
Anti-E Protein Polyclonal Antibody (VK672014) is a rabbit polyclonal antibody detecting E Protein in ELISA, IHC, WB.
Highlights
  • Affinity Purified — Minimal background and high purity for reliable results.
  • Multi-Application — Validated across multiple applications.
Species reactivitySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)
ApplicationsELISA, IHC, WB
Host speciesRabbit
IsotypeIgG
Clonality Polyclonal
Immunogen E. coli - derived recombinant SARS-CoV-2 E Protein (Met1-Val75).
Target Envelope small membrane protein, E, sM protein, E Protein
Purification Purified by antigen affinity column.
Accession P0DTC4
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Envelope small membrane protein is a ~8 kDa protein. Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis. Regulates the localization of S protein at cis-Golgi, the place of virus budding. May act by slowing down the cell secretory pathway.

1. Boson, B. et al. (2021) The Journal of biological chemistry 296, 100111. PMID: 33229438
2. De Maio, F. et al. (2020) Microbes and infection 22, 592-597. PMID: 32891874
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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