HEK293T SARS-CoV-2 Envelope small membrane protein (E) Stable Cell Line (VK672818)

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Overview

Catalog No.	VK672818
Description	HEK293T SARS-CoV-2 Envelope small membrane protein (E) Stable Cell Line (VK672818) is a stable cell line expressing SARS-CoV-2 Envelope small membrane protein (E). Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P0DTC4
Applications	FCM
Species	Viral (SARS-CoV-2)
Host species	HEK293T
Target	SARS-CoV-2 Envelope small membrane protein (E)
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Envelope small membrane protein (SARS-CoV-2 Envelope small membrane protein (E)) is a ~8 kDa protein. Plays a central role in virus morphogenesis and assembly. Acts as a viroporin and self-assembles in host membranes forming pentameric protein-lipid pores that allow ion transport. Also plays a role in the induction of apoptosis. Regulates the localization of S protein at cis-Golgi, the place of virus budding. May act by slowing down the cell secretory pathway. 1. Boson, B. et al. (2021) The Journal of biological chemistry 296, 100111. PMID: 33229438 2. De Maio, F. et al. (2020) Microbes and infection 22, 592-597. PMID: 32891874

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qPCR

Untransfected cells and Transfected cells were tested with qPCR.

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Datasheet

HEK293T SARS-CoV-2 Envelope small membrane protein (E) Stable Cell Line.pdf

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Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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HEK293T SARS-CoV-2 Envelope small membrane protein (E) Stable Cell Line (VK672818)

Overview

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References

Datasheet

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